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A company has deployed a multi-tier application in Azure, where the web tier is hosted on Azure App Service, the application tier is on Azure Virtual Machines, and the database tier is on Azure SQL Database. Users are reporting intermittent connectivity issues when trying to access the application. After investigating, you find that the web tier can communicate with the application tier, but the application tier cannot reach the database tier. What could be the most likely cause of this connectivity issue?
The Network Security Group (NSG) rules on the subnet of the Azure SQL Database are blocking traffic from the application tier.
The Azure SQL Database is configured with a public endpoint that is not accessible from the application tier.
The application tier is experiencing high CPU usage, causing it to drop database connection requests.
The Azure SQL Database is in a different Azure region than the application tier, causing latency issues.

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